Brittin et al. 2021

A multi-scale brain map derived from whole-brain volumetric reconstructions

Christopher A. Brittin, Steven J. Cook, David H. Hall, Scott W. Emmons & Netta Cohen
Nature 591, 105–110
Published: 24 February 2021

Datasets

Summary

Two complete volumetric reconstructions of the C.elegans nerve ring from previously published EMs (White et al. 1986) -adult -larval stage 4 (L4) worm
Two series of EMs span approx the same volume with a length of 36 micrometres, starting in the anterior and ending in the ventral ganglia
The reconstructions of these two nerve rings provide the first complete, nanoscale-resolution datasets of all neuronal membrane contacts of any neuropil.
Presents a "contactome": the set of membrane contacts of the brain
They define two neurons as immediate neighbours if the membranes along their neural processes are physically adjacent in at least one EM.
To characterise synaptic pathways within a spatial context, they integrated volumetric reconstructions with recent re-scoring of synapses on the same L4 and adult worms (Cook et al. 2019) for validation and comparison with other datasets (White et al, (1986); Witvliet et al., (2021))

Data released